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The deletion or inactivation of Parkin down-regulates PGC-1, which in turn leads to the selective degeneration of dopamine neurons in the substantia nigra pars compacta (SNpc)

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Immunofluorescence study After overnight fixation in 4% paraformaldehyde at 4 C, the pancreases dissected from neonatal mice were putted in 10% sucrose for 4 h, 20% sucrose for 8 h, and 30% sucrose for 12 h, sequentially