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Peptides were injected onto a 75 m 2 cm trap column (Thermo Fisher) before re-focusing on an Aurora Elite 75 m 15 cm C18 column (IonOpticks) using a Vanquish Neo UHPLC (Thermo Fisher) connected to an Orbitrap Astral mass spectrometer (Thermo Fisher)

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Additional examinations utilizing ESR assessed the relative potential of GHK in neutralizing OH radicals compared to other antioxidants like carnosine and reduced glutathione (GSH), both recognized for their antioxidant capabilities