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Proteins were extracted from cells 6 days after transduction

Clinical trials, such as the SURMOUNT-1 study for tirzepatide and the STEP trials for semaglutide, show that both are highly effective

For Gs recruitment [100], cells were transfected with 0.5 g miniGs-Venus (a gift from Dr Nevin Lambert, Medical College of Georgia [52]) and 0.5 g GLP-1R-nanoLuc plasmids, and recruitment was quantified by nanoBRET 24 h after transfection as above

They are satiety hormones

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