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There were 3 endometrial biopsies (2 Cases, and 1 Control) that did not yield a PCR product for the 16S rRNA gene and were thus considered negative (the band at ~ 80 bp represents dimers of primers + Illumina adapters/barcode/priming region)

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The organic phase, which is at no more than 50C, is distilled in vacuo (to LOD no more than 10% and water content, by KF (Karl Fischer) titration, no more than 0.5%), affording the title compound as an oil of about 85-95% purity, exclusive of toluene

While the needle is still in the vial, turn it upside down so that the vials bottom is facing upwards toward the ceiling

A great storage environment can't protect against poor aseptic technique