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Transfection HEK293 cells were transfected with purified DNAs including pEGFP-C1, pEGFP-N3-MIM I-BAR, pHalo7-C1, pHalo7-C1-MIM I-BAR, pEGFP-C1-GST-Rac1, pHalo7-C1-Rac1 WT and mutants (G12V and T17N), pmCherry-FKBP-MIM I-BAR, pQH-CD63-sfGFP-FKBP, pKLV2-AsCas12fHKRA-Halo7-GSGS-FRB-guideRNA, and pKLV2-AsCas12fHKRA-Halo7-GSGS-FRB-T2A-FKBP-MIM I-BAR-guideRNA using polyethylenimine MAX (PEI, Polysciences, #49553-93-7)

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Based on this, Biomimetic Nanosystems are used to enhance the bioavailability of BBR: a