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Acknowledgements Authors thank Qiong Huang, Jingyao Chen, Chao Bi and Jiajia Wang from the Core Facilities, Zhejiang University School of Medicine for their technical support

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In this study, metformin did not alter oxidative phosphorylation or glycolysis in normal non-cancer rectal tissue biopsies, importantly suggesting that the anti-metabolic effects of metformin may be tumour tissue-specific, which supports its potential utility as a radiosensitiser in rectal cancer