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Plasmid identity was verified using Sanger sequencing, and the most efficient sgRNA (as determined by anti-GCDH immunoblotting) was used in subsequent experiments (sgRNA sequences are listed in Supplementary Table 1)

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IGF-1 DES is a shorter version of IGF-1 (missing the first 3 amino acids) with a very short half-life of 20 to 30 minutes

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