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For the MTT and neutral red assays, cells were plated at both 0.8 10 5 cells/ml 2.4 10 5 cells/ml to establish seeding density for subsequent experiments

Finally, this unique phenotype offers mechanistic insights: the dissociation between severe adiposity and a favorable cardiometabolic profile suggests that MC4R signaling plays a non-redundant role in integrating energy balance with cardiovascular regulation [2,32,33]