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The exendin-4-mediated induction of pRb S807/811 phosphorylation was further confirmed in the mouse Min6 cells through western blotting and immunofluorescence analysis (Supplementary figures S4A, S4B and S4C)

Although this could be interpreted as indicating no internalization, it is also consistent with studies that show that GIPR internalizes slowly but rapidly recycles to the cell surface in a constitutive manner (Mohammad et al., 2014)